Part:BBa_J72036:Design

{Bmll66} in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll66 {<VSV!}

Wobble mll66/mll67                (67, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll66         {<VSV!}
----
mll66    Forward wobble for {<VSV!} (Bmll66)
CCATAGAATTCATGAGATCTTACACCGATATAGAGATGAACAGGCTGGG
mll67    Reverse wobble for {<VSV!} (Bmll66)
CGTTAGGATCCttaCTTTCCCAGCCTGTTCATCTCTATA